Mercodia kits are based strictly on stable, monoclonal antibodies combined with other top quality reagents. This ensures safe long-term production with minimal lot-to-lot variation. By the careful selection of reagents, Mercodia products provide accurate results by first run.
The links underneath will lead you through the principle of the ELISA technology, the advantages of monoclonal antibodies and the accuracy of Mercodia´s assays. You will learn more about the hook effect and sensitivity and how to calculate your results. And last but not least how you can establish your own controls.
Principle of Technology
ELISA (ENZYME-LINKED IMMUNOSORBENT ASSAY) is a widely used biochemical technique for the detection of an antigen in a sample.
Since the discovery by César Milstein and George Köhler in 1975 on the construction of a continuously growing cell line expressing a specific, predefined monoclonal antibody, it has been possible to produce unlimited amounts of pure and specific monoclonal antibodies.
Sensitivity is a statistical term used to describe the ability of an assay to separate the blank from the specific analyte. Since there are a variety of methods used for determining sensitivity, the method must be known when comparing sensitivity between assays.
The routine analysis of controls with known concentrations is a valuable tool for the acceptance of test runs and to validate that analyzed sample concentrations are in fact repeatable and reproducible at your laboratory.
Diabetes Mellitus is a combination of several different diseases with various etiologies. Diabetes arises when the beta cells of the Langerhans islets in the pancreas is unable to meet insulin demands in the organism, either due to functional failure and destruction of the beta cell mass or because of insulin needs due to insulin resistance or growth that are disproportionate to insulin secretory capacity.
This condition has been known for over 3000 years and today diabetes is one of the major health challenges of the 21st century. This non-communicable disease has reached epidemic proportions in all regions of the world and the prevalence of diabetes is expected to continue to rise inexorably if nothing is done to slow this epidemic. The International Diabetes Federation has predicted that the sufferers from diabetes will exceed 350 million by 2025.
The two most common forms of diabetes are Insulin Dependent Diabetes Mellitus (IDDM), also called Type 1 Diabetes, and Non Insulin Dependent Diabetes Mellitus (NIDDM), also called Type 2 Diabetes.
How to choose your glucagon assay
Inaccurate glucagon readings can have enormous negative implications on research and clinical decisions, in this section we will tell you more about what distinguishes the Mercodia Glucagon ELISAs from all other glucagon ELISAs, and give you some tips on what to look for in a good glucagon assay.
Given the suggested patho-physiological significance of oxLDL, there has been a significant increase in the research of the pathogenic role of circulating oxLDL, giving new insights and questions in regards to the role of oxLDL in cardiovascular disease.